TFIIIC220 Double Nickase Plasmid (h): sc-404920-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFIIIC220 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TFIIIC220 Double Nickase Plasmid (h) and TFIIIC220 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GTF3C1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TFIIIC220 Antibody (F-12): sc-398780
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIIC220 Double Nickase Plasmid (h) | sc-404920-NIC | 20 µg | $410.00 | |||
TFIIIC220 Double Nickase Plasmid (h2) | sc-404920-NIC-2 | 20 µg | $410.00 |
GTF3C1 encodes TFIIIC220, the largest subunit of the TFIIIC complex that recognizes internal promoter elements in RNA polymerase III–transcribed genes, including tRNAs and other small noncoding RNAs. Through promoter binding and recruitment of TFIIIB, TFIIIC220 supports Pol III preinitiation complex assembly and regulates transcriptional output linked to translation capacity, stress adaptation, and cell-cycle progression. TFIIIC also contributes to higher-order genome organization by associating with chromatin and boundary elements, connecting Pol III transcription to nuclear architecture. Dysregulation of Pol III transcriptional control and TFIIIC function is frequently investigated in contexts of altered proliferation programs, genome stability, and transcriptional rewiring observed in cancer and developmental disease models.
TFIIIC220 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GTF3C1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GTF3C1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GTF3C1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GTF3C1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.