TFIIF RAP 74 Double Nickase Plasmid (h): sc-405237-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFIIF RAP 74 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TFIIF RAP 74 Double Nickase Plasmid (h) and TFIIF RAP 74 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GTF2F1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIF RAP 74 Double Nickase Plasmid (h) | sc-405237-NIC | 20 µg | $410.00 | |||
TFIIF RAP 74 Double Nickase Plasmid (h2) | sc-405237-NIC-2 | 20 µg | $410.00 |
GTF2F1 encodes TFIIF RAP 74, a core subunit of the general transcription factor IIF that cooperates with RNA polymerase II to support preinitiation complex formation, promoter escape, and early elongation. Through coordination with TFIIB, TFIIE, and TFIIH, TFIIF helps modulate transcription start site usage and influences genome-wide transcriptional programs tied to cell-cycle control and stress-responsive gene expression. Disruption of Pol II transcription factor networks, including components of TFIIF, is frequently studied in the context of transcriptional dysregulation, replication stress, and altered proteostasis observed across diverse disease models. GTF2F1 is therefore a useful node for interrogating transcription machinery dependencies and their downstream effects on chromatin organization and signaling pathways.
TFIIF RAP 74 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GTF2F1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GTF2F1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GTF2F1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GTF2F1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.