TCR delta Antikörper (H-41) Empfohlen für die Detektion von TCR δ aus der Spezies human per WB, IP und ELISA

TCR δ Antikörper (H-41): sc-100289

TCR δ Antikörper (H-41) wurde bewertet mit 4.6 von 5 von 5.
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Datenblatt

    • Anti-TCR delta Antikörper H-41 ist ein monoklonales IgG1 (kappa light chain) TCR δ Antikörper in einer Menge von 100 µg/ml
    • gezogen gegen rekombinantes TCR δ aus der Spezies human
    • Empfohlen für die Detektion von TCR δ aus der Spezies human per WB, IP und ELISA
    • Beachten Sie m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), unsere besonders zu empfehlenden rekombinanten Alternativen zu konventionellen sekundären anti-Maus IgG Reagenzien.
    • Bitte kontaktieren Sie uns um ein 10 µg GRATIS Sample vom TCR δ (H-41): sc-100289 zu erhalten.

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Gefragt von: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Beantwortet von: Technical Service 11
Veröffentlichungsdatum: 2017-02-03
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Rated 5 von 5 von aus Very good TCRgd+ cell staining on FFPE After TCR gamma.20 (from Thermo Scientific) being discontinued, finally I've found in Santa Cruz Biotechnology TCR delta a solid antibody useful to recognize TCRgd cells in formalin-fixed paraffin embedded sample using immunohistochemistry. I've stained sections from tonsil and small intestine with a range of dilution from 1:25 to 1:250 and I've achieved strong and tidy stainings in all dilutions tested. Also antigen unmasking is very tolerant. It works using EDTA pH8.0 or Citrate pH 6.0 in microwave or water bath. I'm very satisfied from the results
Veröffentlichungsdatum: 2020-03-12
Rated 5 von 5 von aus Excellent staining for IHC on FFPE tissues I successfully used this product for immunohistochemistry on 4 micron sections of FFPE tonsil tissues and FFPE cell block of a bone marrow blood clot. I got excellent results using 1:50-1:100 dilution for 1 hour incubation at room temperature manually or using the Ventena Benchmark Ultra autostainer. The best results were obtained using EDTA pH9 in a steamer then placed on the autostainer, or alternately using CC1 at 99 degrees C for 36 minutes. Ventena Ultraview/DAB was used for the detection system.
Veröffentlichungsdatum: 2018-06-19
Rated 5 von 5 von aus Clean and Great expression of TCR Delta. I struggled for over a year to find a replacement for TCR Gamma, which was discontinued by Thermo last year. This is a great replacement for it. I can't upload a TIFF image, but the examples that I wanted to upload were from a Spleen and Colon. The retrieval for Leica Bond III was Hier2 for 40 minutes with 15 minutes of antibody incubation (modified F Protocol). Dilution that worked best was 1:200 (using Leica's diluent).
Veröffentlichungsdatum: 2017-12-20
Rated 5 von 5 von aus Good for human detection Positive signal obtained at predicted molecular weight in WB for TCR delta.
Veröffentlichungsdatum: 2017-02-07
Rated 3 von 5 von aus Faint band at correct MW in HeLa whole cell lysate Faint band at correct MW in HeLa whole cell lysate. -SCBT QC
Veröffentlichungsdatum: 2013-12-12
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