TALK-1 CRISPR/Cas9 KO Plasmid (h): sc-406360
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TALK-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TALK-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TALK-1 CRISPR/Cas9 KO Plasmid (h) | sc-406360 | 20 µg | $397.00 |
Overview
KCNK16 encodes TALK-1 (K2P16.1), a two-pore domain potassium channel that contributes to background K⁺ conductance and sets the resting membrane potential in excitable and secretory cells. By regulating membrane excitability, TALK-1 influences voltage-dependent Ca²⁺ entry and downstream Ca²⁺-coupled signaling pathways that shape stimulus–secretion coupling and cellular stress responses. The channel is enriched in pancreatic islets, where altered K⁺ flux can modulate β-cell electrical activity and insulin release dynamics. Genetic and functional studies have linked KCNK16/TALK-1 activity to metabolic traits, making it relevant for mechanistic research in diabetes-associated signaling and ion channel physiology.
TALK-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCNK16 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCNK16 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCNK16 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TALK-1 protein expression.
This CRISPR knockout system enables efficient generation of KCNK16-deficient cell models for investigation of TALK-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCNK16 exon(s) critical for TALK-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCNK16 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TALK-1 CRISPR/Cas9 KO Plasmid (h) and TALK-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCNK16 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TALK-1 HDR Plasmid (h) and TALK-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCNK16 homology arms to support homology-directed repair at defined KCNK16 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.