Synaptogyrin-2 CRISPR/Cas9 KO Plasmid (m): sc-423239
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synaptogyrin-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Synaptogyrin-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synaptogyrin-2 CRISPR/Cas9 KO Plasmid (m) | sc-423239 | 20 µg | $397.00 |
Overview
Syngr2 encodes synaptogyrin-2, a tetraspan synaptic vesicle membrane protein enriched in presynaptic terminals where it contributes to vesicle organization and neurotransmitter release dynamics. Synaptogyrin-2 participates in the synaptic vesicle cycle, influencing exocytosis/endocytosis coupling and vesicle trafficking within neuronal and neuroendocrine cells. Through its role in regulating vesicle availability and release probability, SYNG2-related mechanisms are relevant to studies of synaptic plasticity and circuit function. Altered presynaptic vesicle handling is a recurring feature in models of neurodevelopmental and neurodegenerative disorders, making Syngr2 a useful target for mechanistic interrogation of synaptic dysfunction.
Synaptogyrin-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Syngr2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Syngr2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Syngr2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Synaptogyrin-2 protein expression.
This CRISPR knockout system enables efficient generation of Syngr2-deficient cell models for investigation of Synaptogyrin-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Syngr2 exon(s) critical for Synaptogyrin-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Syngr2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Synaptogyrin-2 CRISPR/Cas9 KO Plasmid (m) and Synaptogyrin-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Syngr2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Synaptogyrin-2 HDR Plasmid (m) and Synaptogyrin-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Syngr2 homology arms to support homology-directed repair at defined Syngr2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.