Svs4 CRISPR/Cas9 KO Plasmid (m): sc-423226
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Svs4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Svs4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Svs4 CRISPR/Cas9 KO Plasmid (m) | sc-423226 | 20 µg | $397.00 |
Overview
Svs4 (seminal vesicle secretory 4) encodes a male reproductive tract secreted protein enriched in the seminal vesicle and associated with the composition and functional properties of seminal fluid. Svs4 contributes to post-mating processes by influencing semen coagulation dynamics, sperm transport, and the biochemical milieu that supports sperm survival and capacitation. Its expression is tightly linked to androgen-regulated secretory programs and luminal epithelial differentiation in accessory sex glands. Altered regulation of seminal vesicle secretory proteins is used as a readout for endocrine disruption and inflammatory or degenerative changes in male reproductive tissues, making Svs4 a useful marker in mechanistic studies of fertility-related phenotypes.
Svs4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Svs4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Svs4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Svs4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Svs4 protein expression.
This CRISPR knockout system enables efficient generation of Svs4-deficient cell models for investigation of Svs4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Svs4 exon(s) critical for Svs4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Svs4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Svs4 CRISPR/Cas9 KO Plasmid (m) and Svs4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Svs4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Svs4 HDR Plasmid (m) and Svs4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Svs4 homology arms to support homology-directed repair at defined Svs4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.