Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SSRP1 CRISPR/Cas9 KO Plasmid (h): sc-401780
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SSRP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SSRP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SSRP1 Antibody (D-7): sc-74536
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSRP1 CRISPR/Cas9 KO Plasmid (h) | sc-401780 | 20 µg | $397.00 |
Overview
SSRP1 (structure specific recognition protein 1) is an essential subunit of the FACT chromatin remodeling complex that facilitates nucleosome disassembly and reassembly during transcription, DNA replication, and DNA repair. By coordinating histone chaperone activity with RNA polymerase II progression and replication fork dynamics, SSRP1 helps maintain genome stability and regulates chromatin accessibility. SSRP1-dependent processes intersect with cell-cycle control, DNA damage responses, and epigenetic regulation, making it relevant to studies of replication stress and aberrant transcriptional programs. Dysregulated FACT/SSRP1 activity has been implicated in oncogenic chromatin states and altered proliferative capacity, supporting its use as a mechanistic node in cancer biology and genome maintenance research.
SSRP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SSRP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SSRP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SSRP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SSRP1 protein expression.
This CRISPR knockout system enables efficient generation of SSRP1-deficient cell models for investigation of SSRP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SSRP1 exon(s) critical for SSRP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SSRP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SSRP1 CRISPR/Cas9 KO Plasmid (h) and SSRP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SSRP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SSRP1 HDR Plasmid (h) and SSRP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SSRP1 homology arms to support homology-directed repair at defined SSRP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.