Spartin Double Nickase Plasmid (h): sc-407769-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Spartin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Spartin Double Nickase Plasmid (h) and Spartin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SPG20. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Spartin Antibody (D-4): sc-271888
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Spartin Double Nickase Plasmid (h) | sc-407769-NIC | 20 µg | $410.00 | |||
Spartin Double Nickase Plasmid (h2) | sc-407769-NIC-2 | 20 µg | $410.00 |
SPG20 encodes Spartin, a cytosolic protein implicated in endosomal trafficking and membrane dynamics through interactions with components of the ESCRT machinery and ubiquitin-dependent sorting pathways. Spartin has been linked to regulation of lipid droplet turnover and mitochondrial homeostasis, integrating with cellular quality-control processes that influence proteostasis and organelle function. Disruption of SPG20 is associated with Troyer syndrome, a complicated hereditary spastic paraplegia characterized by neurodevelopmental and motor system involvement, making Spartin a useful node for studying neuronally relevant trafficking and stress-response mechanisms. In cell models, SPG20 perturbation can be used to interrogate how altered endolysosomal routing and organelle maintenance impact signaling and cellular viability.
Spartin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SPG20 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SPG20. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SPG20 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SPG20-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.