Sox-3 CRISPR/Cas9 KO Plasmid (h): sc-403896
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sox-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sox-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sox-3 Antibody (16-C2): sc-101155
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sox-3 CRISPR/Cas9 KO Plasmid (h) | sc-403896 | 20 µg | $397.00 |
Overview
SOX3 encodes Sox-3, an HMG-box transcription factor that binds DNA and shapes chromatin-dependent gene expression programs essential for early neuroectoderm specification and maintenance of neural progenitor identity. Sox-3 functions within core developmental regulatory networks alongside other SOX family factors, influencing pathways that govern cell fate decisions, proliferation, and differentiation in the developing central nervous system and pituitary axis. Altered SOX3 dosage or regulatory disruption has been associated with neurodevelopmental phenotypes and disorders of hypothalamic–pituitary development, highlighting its relevance to studies of transcriptional control during human development. In research settings, SOX3 is frequently examined for its role in lineage commitment, neural differentiation models, and transcription factor network wiring.
Sox-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SOX3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SOX3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SOX3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sox-3 protein expression.
This CRISPR knockout system enables efficient generation of SOX3-deficient cell models for investigation of Sox-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SOX3 exon(s) critical for Sox-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SOX3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sox-3 CRISPR/Cas9 KO Plasmid (h) and Sox-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SOX3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sox-3 HDR Plasmid (h) and Sox-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SOX3 homology arms to support homology-directed repair at defined SOX3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.