Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SNFT CRISPR Activation Plasmid (h): sc-403344-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNFT CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- SNFT CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by SNFT CRISPR Activation Plasmid (h) and SNFT CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the BATF3 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNFT Antibody (D-6): sc-398902
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNFT CRISPR Activation Plasmid (h) | sc-403344-ACT | 20 µg | $397.00 | |||
SNFT CRISPR Activation Plasmid (h2) | sc-403344-ACT-2 | 20 µg | $397.00 |
BATF3 encodes a basic leucine zipper transcription factor that regulates gene expression programs linked to immune lineage specification and stimulus-responsive transcription. In human cells, BATF3 participates in transcriptional networks downstream of cytokine and pattern-recognition signaling, influencing antigen processing/presentation, interferon-associated responses, and broader inflammatory pathways. Perturbation of BATF3-dependent programs has been associated with altered immune surveillance and dysregulated inflammatory states, making it relevant to studies of tumor–immune interactions and infection-related immunopathology. As a transcriptional regulator, BATF3 is commonly investigated for its effects on cell fate decisions, chromatin-associated gene regulation, and context-dependent responses to microenvironmental cues.
SNFT CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous BATF3 expression without altering the underlying DNA sequence.
SNFT CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the BATF3 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the BATF3 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous SNFT expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native BATF3 locus and enabling the study of SNFT-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of SNFT pathway restoration in tumor cells with silenced or reduced BATF3 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.