SLK Double Nickase Plasmid (h): sc-406490-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLK Double Nickase Plasmid (h) and SLK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SLK Antibody (G-9): sc-515493
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLK Double Nickase Plasmid (h) | sc-406490-NIC | 20 µg | $410.00 | |||
SLK Double Nickase Plasmid (h2) | sc-406490-NIC-2 | 20 µg | $410.00 |
Human SLK (STE20-like serine/threonine kinase) is a cytoplasmic kinase implicated in cytoskeletal remodeling, cell polarity, and regulation of microtubule- and actin-associated dynamics. It participates in signaling networks that coordinate cell migration, adhesion, and stress-responsive pathways, and has been linked to modulation of apoptotic and proliferative programs in a context-dependent manner. SLK activity intersects with kinase cascades that influence mitotic progression and tissue morphogenesis, making it relevant to studies of epithelial integrity and invasive phenotypes. Dysregulation of SLK expression or signaling has been associated in the literature with oncogenic processes, metastasis-related traits, and altered neuronal and developmental biology, supporting its utility as a target for mechanistic investigation.
SLK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.