SLITRK4 CRISPR/Cas9 KO Plasmid (h): sc-414460
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLITRK4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SLITRK4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLITRK4 CRISPR/Cas9 KO Plasmid (h) | sc-414460 | 20 µg | $397.00 |
Overview
SLITRK4 encodes a neuronal transmembrane protein in the SLITRK family implicated in neurite outgrowth, synapse development, and cell–cell adhesion processes that shape neural circuit formation. Through extracellular leucine-rich repeat motifs and intracellular signaling interfaces, SLITRK4 is linked to pathways governing synaptogenesis, axon/dendrite patterning, and regulation of neuronal connectivity. Expression patterns in the nervous system and functional homology across SLITRK proteins support a role in neurodevelopmental and neuropsychiatric-relevant biology. Altered SLITRK-family activity has been investigated in the context of circuit-level dysfunction, making SLITRK4 a useful target for mechanistic studies in neuronal models.
SLITRK4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLITRK4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLITRK4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLITRK4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SLITRK4 protein expression.
This CRISPR knockout system enables efficient generation of SLITRK4-deficient cell models for investigation of SLITRK4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLITRK4 exon(s) critical for SLITRK4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLITRK4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SLITRK4 CRISPR/Cas9 KO Plasmid (h) and SLITRK4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLITRK4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SLITRK4 HDR Plasmid (h) and SLITRK4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLITRK4 homology arms to support homology-directed repair at defined SLITRK4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.