Slit1 Antibody (G-4): sc-376756
- Slit1 Antibody (G-4) is a mouse monoclonal IgG1 κ Slit1 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1391-1527 mapping near the C-terminus of Slit1 of human origin
- Slit1 Antibody (G-4) is recommended for detection of Slit1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Slit1 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Slit1 Antibody (G-4). This work is in progress.
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Slit1 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Slit1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Slit1 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Slit1 plays a crucial role in neural development, particularly in axonal guidance, by serving as a high-affinity signaling ligand for the Roundabout (Robo) receptor, which is essential for directing the growth of axons during the formation of the nervous system. Slit1 is secreted and belongs to a family of leucine-rich repeat-containing glycoproteins (Slit1-3) that are expressed in the midline of the central nervous system across various vertebrate species, indicating a conserved function in regulating mesodermal cell movement during gastrulation. Notably, Slit1 is involved in the short-range inhibitory guidance of retinal ganglion cell axons, which is vital for the spatial organization of these cells during development. Slit1′s interaction with Robo receptors highlights its importance in establishing proper neural connections, making Slit1 a significant target for research in neurodevelopmental processes and potential therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Slit1 Antibody (G-4) References:
- Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance. | Brose, K., et al. 1999. Cell. 96: 795-806. PMID: 10102268
- Distinct but overlapping expression patterns of two vertebrate slit homologs implies functional roles in CNS development and organogenesis. | Holmes, GP., et al. 1998. Mech Dev. 79: 57-72. PMID: 10349621
- Retinal ganglion cell axon guidance in the mouse optic chiasm: expression and function of robos and slits. | Erskine, L., et al. 2000. J Neurosci. 20: 4975-82. PMID: 10864955
- slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains. | Rothberg, JM., et al. 1990. Genes Dev. 4: 2169-87. PMID: 2176636
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Slit1 Antibody (G-4) | sc-376756 | 200 µg/ml | $322.00 | |||
Slit1 Antibody (G-4) AC | sc-376756 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Slit1 Antibody (G-4) HRP | sc-376756 HRP | 200 µg/ml | $322.00 | |||
Slit1 Antibody (G-4) FITC | sc-376756 FITC | 200 µg/ml | $336.00 | |||
Slit1 Antibody (G-4) PE | sc-376756 PE | 200 µg/ml | $349.00 | |||
Slit1 Antibody (G-4) Alexa Fluor® 488 | sc-376756 AF488 | 200 µg/ml | $364.00 | |||
Slit1 Antibody (G-4) Alexa Fluor® 546 | sc-376756 AF546 | 200 µg/ml | $364.00 | |||
Slit1 Antibody (G-4) Alexa Fluor® 594 | sc-376756 AF594 | 200 µg/ml | $364.00 | |||
Slit1 Antibody (G-4) Alexa Fluor® 647 | sc-376756 AF647 | 200 µg/ml | $364.00 | |||
Slit1 Antibody (G-4) Alexa Fluor® 680 | sc-376756 AF680 | 200 µg/ml | $364.00 | |||
Slit1 Antibody (G-4) Alexa Fluor® 790 | sc-376756 AF790 | 200 µg/ml | $364.00 |