Shank 1 Double Nickase Plasmid (h): sc-403064-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Shank 1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Shank 1 Double Nickase Plasmid (h) and Shank 1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SHANK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Shank 1 Antibody (E-1): sc-398630
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Shank 1 Double Nickase Plasmid (h) | sc-403064-NIC | 20 µg | $410.00 | |||
Shank 1 Double Nickase Plasmid (h2) | sc-403064-NIC-2 | 20 µg | $410.00 |
SHANK1 encodes Shank 1, a multidomain scaffolding protein enriched at excitatory postsynaptic densities where it organizes receptors, ion channels, and actin-regulatory complexes to shape synaptic architecture and signaling. Through interactions with PSD proteins and cytoskeletal regulators, Shank 1 helps couple glutamatergic receptor complexes to downstream pathways governing synapse maturation, spine morphogenesis, and activity-dependent plasticity. Altered SHANK family function has been linked to disrupted excitatory synapse organization and neurodevelopmental phenotypes, making SHANK1 a useful node for studying mechanisms that impact circuit connectivity. Human SHANK1 is therefore widely investigated in neuronal differentiation models and synaptogenesis assays to connect scaffolding dynamics with functional synaptic outputs.
Shank 1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SHANK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SHANK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SHANK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SHANK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.