RYK CRISPR/Cas9 KO Plasmid (h): sc-402654
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RYK CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RYK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RYK Antibody (F35 P7 D7 F5): sc-83082
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RYK CRISPR/Cas9 KO Plasmid (h) | sc-402654 | 20 µg | $397.00 |
Overview
RYK (receptor-like tyrosine kinase) encodes an atypical single-pass transmembrane receptor that functions primarily as a co-receptor in non-canonical WNT signaling, including WNT5A-driven pathways that regulate cell polarity, directional migration, and cytoskeletal dynamics. Through interactions with WNT ligands and Frizzled receptors, RYK can influence planar cell polarity and downstream effectors such as JNK, linking extracellular cues to transcriptional and morphogenetic programs. RYK activity has been associated with neural development and axon guidance, and dysregulated RYK/WNT signaling has been reported in contexts of tumor cell invasion, metastasis-associated phenotypes, and inflammatory microenvironments. These properties make RYK a useful target for probing pathway crosstalk between WNT signaling, cell adhesion, and migratory behavior in human cell models.
RYK CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RYK gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RYK together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RYK open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RYK protein expression.
This CRISPR knockout system enables efficient generation of RYK-deficient cell models for investigation of RYK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RYK exon(s) critical for RYK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RYK genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RYK CRISPR/Cas9 KO Plasmid (h) and RYK CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RYK locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RYK HDR Plasmid (h) and RYK HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RYK homology arms to support homology-directed repair at defined RYK target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.