RP105 Antibody (RP/14): sc-13592

RP105 Antibody (RP/14) is a rat monoclonal IgG2a antibody that detects the RP105 protein of mouse origin by immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). RP105 monoclonal antibody (RP/14) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RP105 protein is a critical surface molecule on mouse B cells, playing a vital role in transmitting growth-promoting signals that influence the life and death decisions of these immune cells. Notably, RP105 features tandem repeats of a leucine-rich motif in its extracellular domain, which are essential for mediating protein-protein interactions, thereby facilitating communication between B cells and their environment. The amino acid sequence of human RP105 shows a high degree of homology to that of mouse RP105, sharing 74% identity, including the conserved leucine-rich motif, which underscores the evolutionary significance of this protein in immune function. The expression of RP105 is notably enhanced in the presence of MD1, although specifically restricted to CD19-positive B cells, with predominant expression observed on mature B cells located in the mantle zones, while minimal expression is found in germinal centers. This specificity and expression pattern highlight the importance of RP105 in B cell maturation and function, making RP105 (RP/14) antibody an invaluable tool for researchers studying B cell biology and related immunological processes.

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RP105 Antibody (RP/14) References:

1. Host defense mechanisms triggered by microbial lipoproteins through toll-like receptors.  |  Brightbill, HD., et al. 1999. Science. 285: 732-6. PMID: 10426995
2. Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9.  |  Chuang, TH. and Ulevitch, RJ. 2000. Eur Cytokine Netw. 11: 372-8. PMID: 11022120
3. Innate recognition of lipopolysaccharide by Toll-like receptor 4/MD-2 and RP105/MD-1.  |  Miyake, K., et al. 2000. J Endotoxin Res. 6: 389-91. PMID: 11521060
4. Difference in B cell activation between dermatomyositis and polymyositis: analysis of the expression of RP105 on peripheral blood B cells.  |  Kikuchi, Y., et al. 2001. Ann Rheum Dis. 60: 1137-40. PMID: 11709456
5. Drosophila Toll and IL-1 receptor.  |  Gay, NJ. and Keith, FJ. 1991. Nature. 351: 355-6. PMID: 1851964
6. Molecular cloning of a human RP105 homologue and chromosomal localization of the mouse and human RP105 genes (Ly64 and LY64).  |  Miura, Y., et al. 1996. Genomics. 38: 299-304. PMID: 8975706
7. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity.  |  Medzhitov, R., et al. 1997. Nature. 388: 394-7. PMID: 9237759
8. A family of human receptors structurally related to Drosophila Toll.  |  Rock, FL., et al. 1998. Proc Natl Acad Sci U S A. 95: 588-93. PMID: 9435236
9. Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression.  |  Miyake, K., et al. 1998. J Immunol. 161: 1348-53. PMID: 9686597
10. RP105 is associated with MD-1 and transmits an activation signal in human B cells.  |  Miura, Y., et al. 1998. Blood. 92: 2815-22. PMID: 9763566

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RP105 Antibody (RP/14) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-RP105 Antibody (RP/14) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RP105 Antibody (RP/14) Specifications

Alternate Product Name: RP105

Product Clone Name: RP/14

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: RP105

Epitope Species: mouse

Product Reactivity/Detection: RP105

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for RP105 Antibody (RP/14): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin