Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
robo2 CRISPR/Cas9 KO Plasmid (m): sc-435289
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- robo2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the robo2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: robo2 Antibody (A-10): sc-376177
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
robo2 CRISPR/Cas9 KO Plasmid (m) | sc-435289 | 20 µg | $397.00 |
Overview
ROBO2 (Roundabout guidance receptor 2) encodes a transmembrane receptor in the SLIT/ROBO signaling axis that regulates axon guidance, neuronal migration, and tissue morphogenesis through cytoskeletal remodeling and directional cell movement. In mouse, robo2 function is linked to development of the nervous system and multiple epithelial structures, with downstream effects on pathways controlling cell polarity, adhesion, and repulsive guidance cues. Altered ROBO2 activity has been associated with congenital developmental abnormalities and dysregulated cell migration phenotypes, making it relevant for mechanistic studies of neurodevelopment and organogenesis. Its signaling interfaces with Rho family GTPase-dependent processes that shape growth cone dynamics and coordinated cellular positioning.
robo2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Robo2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Robo2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Robo2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish robo2 protein expression.
This CRISPR knockout system enables efficient generation of Robo2-deficient cell models for investigation of robo2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Robo2 exon(s) critical for robo2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Robo2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by robo2 CRISPR/Cas9 KO Plasmid (m) and robo2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Robo2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by robo2 HDR Plasmid (m) and robo2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Robo2 homology arms to support homology-directed repair at defined Robo2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.