RMND5A CRISPR/Cas9 KO Plasmid (h): sc-413041
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RMND5A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RMND5A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RMND5A CRISPR/Cas9 KO Plasmid (h) | sc-413041 | 20 µg | $397.00 |
Overview
RMND5A (Required for Meiotic Nuclear Division 5 Homolog A) encodes a conserved protein implicated in ubiquitin-dependent protein quality control, with reported roles in E3 ubiquitin ligase-associated processes that influence proteostasis and cell-cycle progression. Through connections to ubiquitination and turnover of regulatory proteins, RMND5A is studied in the context of stress responses, proliferation control, and maintenance of cellular homeostasis. Perturbation of ubiquitin pathway components is frequently linked to genome instability and altered growth signaling, making RMND5A a useful target for mechanistic studies of dysregulated proteostasis in human disease biology.
RMND5A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RMND5A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RMND5A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RMND5A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RMND5A protein expression.
This CRISPR knockout system enables efficient generation of RMND5A-deficient cell models for investigation of RMND5A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RMND5A exon(s) critical for RMND5A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RMND5A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RMND5A CRISPR/Cas9 KO Plasmid (h) and RMND5A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RMND5A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RMND5A HDR Plasmid (h) and RMND5A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RMND5A homology arms to support homology-directed repair at defined RMND5A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.