Rim2 Antibody (63-M7): sc-100842

Rim2 Antibody (63-M7) is a mouse monoclonal IgM antibody that detects Rim2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Rim2 antibody (63-M7) is available in a non-conjugated form, allowing for versatile experimental use. Rim2 plays a crucial role in the regulation of synaptic vesicle exocytosis, which is essential for neurotransmitter release and communication between neurons. Rim2 interacts with Rab3, a key player in calcium-regulated exocytosis, to form a GTP-dependent complex that facilitates the fusion of synaptic vesicles with the plasma membrane. This interaction is vital for maintaining proper synaptic function and ensuring efficient neurotransmission. Rim2 localizes at the plasma membrane of insulin-secreting cells, where Rim2 enhances glucose-stimulated insulin secretion, highlighting its importance in metabolic regulation. The presence of distinct structural domains within Rim2, including a zinc-finger motif and PDZ and C2 domains, further underscores its functional versatility and ability to interact with various signaling pathways. Rim2 monoclonal antibody (63-M7) enables researchers to gain valuable insights into the mechanisms of synaptic transmission and the role of Rim2 in neuroendocrine signaling.

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Rim2 Antibody (63-M7) References:

1. Disruption of Rab3-calmodulin interaction, but not other effector interactions, prevents Rab3 inhibition of exocytosis.  |  Coppola, T., et al. 1999. EMBO J. 18: 5885-91. PMID: 10545100
2. The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins.  |  Wang, Y., et al. 2000. J Biol Chem. 275: 20033-44. PMID: 10748113
3. The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis.  |  Iezzi, M., et al. 2000. FEBS Lett. 474: 66-70. PMID: 10828453
4. cAMP-GEFII is a direct target of cAMP in regulated exocytosis.  |  Ozaki, N., et al. 2000. Nat Cell Biol. 2: 805-11. PMID: 11056535
5. A direct inhibitory role for the Rab3-specific effector, Noc2, in Ca2+-regulated exocytosis in neuroendocrine cells.  |  Haynes, LP., et al. 2001. J Biol Chem. 276: 9726-32. PMID: 11134008
6. Rim is a putative Rab3 effector in regulating synaptic-vesicle fusion.  |  Wang, Y., et al. 1997. Nature. 388: 593-8. PMID: 9252191

The preferred Rim1/2 antibody is Rim1/2 Antibody (B-4): sc-515918.