Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rfp2 CRISPR/Cas9 KO Plasmid (h): sc-403870
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rfp2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rfp2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rfp2 Antibody (E-6): sc-393257
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rfp2 CRISPR/Cas9 KO Plasmid (h) | sc-403870 | 20 µg | $397.00 |
Overview
TRIM13 encodes the human RING finger E3 ubiquitin ligase Rfp2, a predominantly endoplasmic reticulum–associated protein implicated in ubiquitin-dependent proteostasis and ER quality control. Rfp2 participates in regulating protein turnover and stress-responsive signaling by promoting substrate ubiquitylation, linking it to processes such as ER-associated degradation, apoptosis regulation, and innate immune pathway modulation. Altered TRIM13 expression or activity has been studied in contexts of cellular stress adaptation and dysregulated ubiquitin signaling that can contribute to oncogenic phenotypes and neurodegenerative proteinopathies. As part of the TRIM family, Rfp2 provides a tractable node for dissecting ubiquitin pathway wiring and organelle-localized signaling in human cells.
Rfp2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TRIM13 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TRIM13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TRIM13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rfp2 protein expression.
This CRISPR knockout system enables efficient generation of TRIM13-deficient cell models for investigation of Rfp2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TRIM13 exon(s) critical for Rfp2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TRIM13 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rfp2 CRISPR/Cas9 KO Plasmid (h) and Rfp2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TRIM13 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rfp2 HDR Plasmid (h) and Rfp2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TRIM13 homology arms to support homology-directed repair at defined TRIM13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.