RDH10 Antibody (F-6): sc-514121
- RDH10 Antibody (F-6) is a mouse monoclonal IgG2b κ RDH10 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 280-295 within an internal region of RDH10 of human origin
- RDH10 Antibody (F-6) is recommended for detection of RDH10 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RDH10 Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RDH10 Antibody (F-6) for WB applications. These reagents are now offered in bundles with RDH10 Antibody (F-6) (see ordering information below).
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RDH10 Antibody (F-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects RDH10 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RDH10 (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RDH10, also known as retinol dehydrogenase 10, is a 341 amino acid single-pass membrane protein that plays a crucial role in the photic visual cycle by converting all-trans retinol into all-trans retinal, which is essential for vision. This enzymatic activity is particularly important during early embryonic development, as RDH10 is vital for generating retinoic acid, a key signaling molecule involved in cellular differentiation and organogenesis. RDH10 is predominantly located in the retina, but is also expressed in the kidney, liver, small intestine, placenta, lung, heart, and skeletal muscle, highlighting diverse physiological roles. RDH10 shares 100% amino acid identity with the bovine Rdh10 protein and 98.6% with the murine counterpart, indicating a high degree of evolutionary conservation. RDH10 interacts physically with CRALBP and RPE65 in retinal pigment epithelial cells, further underscoring significance in visual processes. The RDH10 gene is located on human chromosome 8q21.11 and is conserved across several species, including chimpanzees, dogs, cows, mice, rats, chickens, and zebrafish, reflecting fundamental biological importance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RDH10 Antibody (F-6) References:
- Cloning and characterization of a novel all-trans retinol short-chain dehydrogenase/reductase from the RPE. | Wu, BX., et al. 2002. Invest Ophthalmol Vis Sci. 43: 3365-72. PMID: 12407145
- Genomic organization and transcription of the human retinol dehydrogenase 10 (RDH10) gene. | Picozzi, P., et al. 2003. FEBS Lett. 554: 59-66. PMID: 14596915
- Screening genes of the retinoid metabolism: novel LRAT mutation in leber congenital amaurosis. | Sénéchal, A., et al. 2006. Am J Ophthalmol. 142: 702-4. PMID: 17011878
- Forced expression of RDH10 gene retards growth of HepG2 cells. | Rossi, E., et al. 2007. Cancer Biol Ther. 6: 238-44. PMID: 17218779
- RDH10 is essential for synthesis of embryonic retinoic acid and is required for limb, craniofacial, and organ development. | Sandell, LL., et al. 2007. Genes Dev. 21: 1113-24. PMID: 17473173
- Kinetic analysis of human enzyme RDH10 defines the characteristics of a physiologically relevant retinol dehydrogenase. | Belyaeva, OV., et al. 2008. J Biol Chem. 283: 20299-308. PMID: 18502750
- Characterization of key residues and membrane association domains in retinol dehydrogenase 10. | Takahashi, Y., et al. 2009. Biochem J. 419: 113-22, 1 p following 122. PMID: 19102727
- The 11-cis-retinol dehydrogenase activity of RDH10 and its interaction with visual cycle proteins. | Farjo, KM., et al. 2009. Invest Ophthalmol Vis Sci. 50: 5089-97. PMID: 19458327
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RDH10 Antibody (F-6) | sc-514121 | 200 µg/ml | $322.00 | |||
RDH10 Antibody (F-6): m-IgG Fc BP-HRP Bundle | sc-531035 | 200 µg Ab; 10 µg BP | $361.00 | |||
RDH10 Antibody (F-6): m-IgGκ BP-HRP Bundle | sc-524612 | 200 µg Ab, 40 µg BP | $361.00 | |||
RDH10 Antibody (F-6) AC | sc-514121 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RDH10 Antibody (F-6) HRP | sc-514121 HRP | 200 µg/ml | $322.00 | |||
RDH10 Antibody (F-6) FITC | sc-514121 FITC | 200 µg/ml | $336.00 | |||
RDH10 Antibody (F-6) PE | sc-514121 PE | 200 µg/ml | $349.00 | |||
RDH10 Antibody (F-6) Alexa Fluor® 488 | sc-514121 AF488 | 200 µg/ml | $364.00 | |||
RDH10 Antibody (F-6) Alexa Fluor® 546 | sc-514121 AF546 | 200 µg/ml | $364.00 | |||
RDH10 Antibody (F-6) Alexa Fluor® 594 | sc-514121 AF594 | 200 µg/ml | $364.00 | |||
RDH10 Antibody (F-6) Alexa Fluor® 647 | sc-514121 AF647 | 200 µg/ml | $364.00 | |||
RDH10 Antibody (F-6) Alexa Fluor® 680 | sc-514121 AF680 | 200 µg/ml | $364.00 | |||
RDH10 Antibody (F-6) Alexa Fluor® 790 | sc-514121 AF790 | 200 µg/ml | $364.00 | |||
RDH10 (F-6) Neutralizing Peptide | sc-514121 P | 100 µg/0.5 ml | $69.00 |