RBM15B Double Nickase Plasmid (h): sc-412040-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RBM15B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RBM15B Double Nickase Plasmid (h) and RBM15B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RBM15B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBM15B Double Nickase Plasmid (h) | sc-412040-NIC | 20 µg | $410.00 |
RBM15B (RNA binding motif protein 15B) is a nuclear RNA-binding protein implicated in post-transcriptional gene regulation, including control of pre-mRNA processing and mRNA stability through interactions with splicing and RNA surveillance machinery. As a member of the RBM15 family, it is linked to regulation of transcript fate and may influence epitranscriptomic signaling networks that modulate RNA metabolism. Altered RNA processing and dysregulated RNA-binding protein activity are recurrent features across cancer and neurodevelopmental and immune-related disorders, making RBM15B a useful node for dissecting disease-relevant gene expression programs. RBM15B perturbation studies help map RNA regulatory circuits affecting cell proliferation, differentiation, and stress responses.
RBM15B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RBM15B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RBM15B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RBM15B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RBM15B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.