PYST2 Double Nickase Plasmid (h): sc-404642-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PYST2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PYST2 Double Nickase Plasmid (h) and PYST2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DUSP7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PYST2 Antibody (D-8): sc-377106
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PYST2 Double Nickase Plasmid (h) | sc-404642-NIC | 20 µg | $410.00 | |||
PYST2 Double Nickase Plasmid (h2) | sc-404642-NIC-2 | 20 µg | $410.00 |
Dual specificity phosphatase 7 (DUSP7), also known as PYST2, is a MAPK-directed phosphatase that preferentially dephosphorylates and inactivates ERK1/2, shaping the amplitude and duration of mitogen-driven signaling. By providing negative feedback within the Ras–Raf–MEK–ERK cascade, DUSP7 helps regulate proliferation, differentiation, and stress-adaptive transcriptional programs. Altered DUSP7 expression or activity has been associated with dysregulated MAPK pathway output in cancers and other conditions where ERK signaling governs cell-fate decisions, making it a useful node for mechanistic studies of signaling plasticity. In human cell models, interrogating DUSP7 function can clarify how phosphatase-mediated attenuation influences downstream effectors, including immediate-early genes and cell-cycle regulators.
PYST2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DUSP7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DUSP7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DUSP7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DUSP7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.