Punc Antibody (F-3): sc-514023
- Punc Antibody (F-3) is a mouse monoclonal IgG1 κ Punc antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 332-349 within an extracellular domain of Punc of human origin
- Punc Antibody (F-3) is recommended for detection of Punc of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Punc Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Punc Antibody (F-3) for WB applications. These reagents are now offered in bundles with Punc Antibody (F-3) (see ordering information below).
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SEE ALSO...
Punc Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Punc protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Punc (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Punc protein, also known as the putative neuronal cell adhesion molecule or IGDCC3 (Immunoglobulin superfamily DCC subclass member 3), is a single-pass type I membrane protein composed of 814 amino acids and belongs to the immunoglobulin superfamily and the DCC family. Notably, Punc exhibits a unique structure featuring four immunoglobulin-like C2-type domains and two fibronectin-type III repeats, which are crucial for its role in axon guidance during neural development. Punc is predominantly expressed in the nervous system and limb buds of developing mouse embryos, although expression significantly decreases by midgestation, highlighting its importance in early developmental processes. The Punc gene is conserved across several species, including chimpanzees, dogs, cows, mice, rats, chickens, and zebrafish, and is located on human chromosome 15q22.31, distal to the critical region for Stereocilin, a gene associated with nonsyndromic recessive deafness.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Punc Antibody (F-3) References:
- Deafness locus DFNB16 is located on chromosome 15q13-q21 within a 5-cM interval flanked by markers D15S994 and D15S132. | Villamar, M., et al. 1999. Am J Hum Genet. 64: 1238-41. PMID: 10090914
- Impaired motor coordination in mice that lack punc. | Yang, W., et al. 2001. Mol Cell Biol. 21: 6031-43. PMID: 11486040
- Analysis of the DNA sequence and duplication history of human chromosome 15. | Zody, MC., et al. 2006. Nature. 440: 671-5. PMID: 16572171
- A new locus for non-syndromal, autosomal recessive, sensorineural hearing loss (DFNB16) maps to human chromosome 15q21-q22. | Campbell, DA., et al. 1997. J Med Genet. 34: 1015-7. PMID: 9429146
- Punc, a novel mouse gene of the immunoglobulin superfamily, is expressed predominantly in the developing nervous system. | Salbaum, JM. 1998. Mech Dev. 71: 201-4. PMID: 9507132
- Genomic structure and chromosomal localization of the mouse gene Punc. | Salbaum, JM. 1999. Mamm Genome. 10: 107-11. PMID: 9922388
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Punc Antibody (F-3) | sc-514023 | 200 µg/ml | $322.00 | |||
Punc Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-531005 | 200 µg Ab; 10 µg BP | $361.00 | |||
Punc Antibody (F-3): m-IgGκ BP-HRP Bundle | sc-524576 | 200 µg Ab, 40 µg BP | $361.00 | |||
Punc Antibody (F-3) AC | sc-514023 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Punc Antibody (F-3) HRP | sc-514023 HRP | 200 µg/ml | $322.00 | |||
Punc Antibody (F-3) FITC | sc-514023 FITC | 200 µg/ml | $336.00 | |||
Punc Antibody (F-3) PE | sc-514023 PE | 200 µg/ml | $349.00 | |||
Punc Antibody (F-3) Alexa Fluor® 488 | sc-514023 AF488 | 200 µg/ml | $364.00 | |||
Punc Antibody (F-3) Alexa Fluor® 546 | sc-514023 AF546 | 200 µg/ml | $364.00 | |||
Punc Antibody (F-3) Alexa Fluor® 594 | sc-514023 AF594 | 200 µg/ml | $364.00 | |||
Punc Antibody (F-3) Alexa Fluor® 647 | sc-514023 AF647 | 200 µg/ml | $364.00 | |||
Punc Antibody (F-3) Alexa Fluor® 680 | sc-514023 AF680 | 200 µg/ml | $364.00 | |||
Punc Antibody (F-3) Alexa Fluor® 790 | sc-514023 AF790 | 200 µg/ml | $364.00 | |||
Punc (F-3) Neutralizing Peptide | sc-514023 P | 100 µg/0.5 ml | $69.00 |