Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PTP1B Double Nickase Plasmid (h): sc-400732-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PTP1B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PTP1B Double Nickase Plasmid (h) and PTP1B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PTPN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PTP1B Antibody (D-4): sc-133259
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PTP1B Double Nickase Plasmid (h) | sc-400732-NIC | 20 µg | $410.00 | |||
PTP1B Double Nickase Plasmid (h2) | sc-400732-NIC-2 | 20 µg | $410.00 |
PTPN1 encodes protein tyrosine phosphatase 1B (PTP1B), an endoplasmic reticulum–associated phosphatase that dephosphorylates activated receptor tyrosine kinases and key adaptor proteins. By attenuating insulin receptor and leptin receptor signaling and modulating JAK/STAT, MAPK/ERK, and PI3K/AKT pathway outputs, PTP1B helps regulate cellular metabolism, growth-factor responsiveness, and inflammatory signaling. Altered PTPN1/PTP1B activity has been linked to dysregulated glucose homeostasis and adiposity, and it is frequently studied in the context of oncogenic signaling networks driven by aberrant tyrosine phosphorylation. In immune and stromal compartments, PTP1B also shapes cytokine signaling dynamics, making it relevant to studies of cellular stress responses and signaling crosstalk.
PTP1B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PTPN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PTPN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PTPN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PTPN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.