Psf3 CRISPR/Cas9 KO Plasmid (h): sc-406717
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Psf3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Psf3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Psf3 CRISPR/Cas9 KO Plasmid (h) | sc-406717 | 20 µg | $397.00 |
Overview
GINS3 encodes Psf3, a core subunit of the heterotetrameric GINS complex that cooperates with CDC45 and the MCM2–7 helicase to form the CMG replicative helicase required for origin firing and processive DNA replication. By supporting replication fork progression and coordination with S-phase checkpoint signaling, Psf3 helps maintain genome stability during cell-cycle transit. Perturbation of GINS complex activity is linked to replication stress, altered proliferative capacity, and DNA damage responses that are frequently leveraged in studies of oncogenic transformation. As a replication factor with cell-cycle–regulated expression, GINS3 is widely used as a molecular entry point for investigating replication dynamics, chromatin-associated replisome assembly, and pathways that safeguard DNA integrity.
Psf3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GINS3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GINS3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GINS3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Psf3 protein expression.
This CRISPR knockout system enables efficient generation of GINS3-deficient cell models for investigation of Psf3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GINS3 exon(s) critical for Psf3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GINS3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Psf3 CRISPR/Cas9 KO Plasmid (h) and Psf3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GINS3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Psf3 HDR Plasmid (h) and Psf3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GINS3 homology arms to support homology-directed repair at defined GINS3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.