PRR14 CRISPR/Cas9 KO Plasmid (m): sc-433320
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRR14 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRR14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRR14 CRISPR/Cas9 KO Plasmid (m) | sc-433320 | 20 µg | $397.00 |
Overview
Prr14 encodes PRR14, a proline-rich nuclear protein implicated in organizing nuclear architecture through interactions with the nuclear lamina and chromatin. PRR14 contributes to tethering heterochromatin to the nuclear periphery, influencing epigenetic state, genome stability, and cell cycle–linked changes in nuclear organization. Altered regulation of nuclear lamina–chromatin contacts has been associated with defects in differentiation programs and aberrant proliferative signaling, making PRR14 a useful node for studying nuclear envelope–dependent gene regulation. In mouse systems, Prr14 perturbation can help interrogate how spatial genome organization interfaces with transcriptional control and stress-responsive pathways.
PRR14 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Prr14 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Prr14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Prr14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRR14 protein expression.
This CRISPR knockout system enables efficient generation of Prr14-deficient cell models for investigation of PRR14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Prr14 exon(s) critical for PRR14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Prr14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRR14 CRISPR/Cas9 KO Plasmid (m) and PRR14 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Prr14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRR14 HDR Plasmid (m) and PRR14 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Prr14 homology arms to support homology-directed repair at defined Prr14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.