PRDM14 Double Nickase Plasmid (h): sc-404426-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRDM14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PRDM14 Double Nickase Plasmid (h) and PRDM14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRDM14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PRDM14 Antibody (F-10): sc-518186
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRDM14 Double Nickase Plasmid (h) | sc-404426-NIC | 20 µg | $410.00 | |||
PRDM14 Double Nickase Plasmid (h2) | sc-404426-NIC-2 | 20 µg | $410.00 |
PRDM14 encodes a PR/SET domain–containing transcriptional regulator that helps establish and maintain pluripotency programs and germ cell competence through epigenetic and transcriptional control. In human cells, PRDM14 modulates chromatin state and DNA methylation landscapes, influencing lineage commitment, reprogramming efficiency, and developmental gene regulatory networks. Aberrant PRDM14 expression and dysregulated pluripotency circuitry have been associated with altered differentiation states and oncogenic transcriptional programs in multiple tumor contexts. As a node in stemness-associated pathways, PRDM14 is frequently interrogated to dissect mechanisms of epigenetic regulation, cell fate decisions, and transcription factor network stability.
PRDM14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRDM14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRDM14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRDM14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRDM14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.