Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
POLDIP3 CRISPR Activation Plasmid (h): sc-413603-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- POLDIP3 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- POLDIP3 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by POLDIP3 CRISPR Activation Plasmid (h) and POLDIP3 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the POLDIP3 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: POLDIP3 Antibody (G-2): sc-398931
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POLDIP3 CRISPR Activation Plasmid (h) | sc-413603-ACT | 20 µg | $397.00 |
Human POLDIP3 (polymerase delta interacting protein 3), also known as SKAR, is a regulatory factor that links mRNA processing to translation and supports efficient expression of spliced transcripts. It participates in RNA metabolism and ribosome-associated processes, including coupling of exon junction complex–associated signaling to translational output, thereby influencing cell growth and stress-responsive gene expression programs. Through these activities, POLDIP3 impacts pathways controlling proliferation and genome maintenance, making it relevant to studies of dysregulated translation and RNA processing observed in cancer and other proliferative or stress-related disorders. Its functional connections to transcriptional and post-transcriptional control also position POLDIP3 as a useful node for dissecting gene expression regulation in human cells.
POLDIP3 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous POLDIP3 expression without altering the underlying DNA sequence.
POLDIP3 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the POLDIP3 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the POLDIP3 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous POLDIP3 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native POLDIP3 locus and enabling the study of POLDIP3-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of POLDIP3 pathway restoration in tumor cells with silenced or reduced POLDIP3 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.