Pit-1 Antibody (2C11): sc-47761

Pit-1 Antibody (2C11) is a mouse monoclonal IgG1 antibody that detects Pit-1 in mouse, rat, human, bovine, and porcine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Pit-1, also known as growth hormone factor-1 (GHF-1), is a crucial transcriptional regulator that plays a significant role in the development of the anterior pituitary gland, where Pit-1 is essential for the differentiation and function of somatotropes, lactotropes, and thyrotropes. Pit-1 features a bipartite DNA binding domain, which is vital for activating the transcription of growth hormone and prolactin, thereby influencing growth and metabolic processes. Additionally, Pit-1 exists in two alternatively spliced forms, Pit-1a and Pit-1b, which exhibit different trans-activation capabilities, further underscoring the complexity of regulatory functions. Mutations in the Pit-1 gene can lead to combined pituitary hormone deficiency (CPHD), affecting the production of growth hormone, prolactin, and thyroid-stimulating hormone, which highlights Pit-1′s critical role in endocrine health. The gene encoding Pit-1 is located on human chromosome 3p11, making Pit-1 a significant target for studies related to pituitary function and associated disorders. Anti-Pit-1 antibody (2C11) is an invaluable tool for researchers investigating the mechanisms of pituitary development and hormone regulation.

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Pit-1 Antibody (2C11) References:

1. Role of a pituitary-specific transcription factor (pit-1/GHF-1) or a closely related protein in cAMP regulation of human thyrotropin-beta subunit gene expression.  |  Steinfelder, HJ., et al. 1992. J Clin Invest. 89: 409-19. PMID: 1310694
2. Absent or delayed adrenarche in Pit-1/POU1F1 deficiency.  |  Taha, D., et al. 2005. Horm Res. 64: 175-9. PMID: 16210857
3. GHRH proliferative action on somatotrophs is cell-type specific and dependent on Pit-1/GHF-1 expression.  |  Solloso, A., et al. 2008. J Cell Physiol. 215: 140-50. PMID: 17941086
4. The homeodomain protein, Pit-1/GHF-1, is capable of binding to and activating cell-specific elements of both the growth hormone and prolactin gene promoters.  |  Fox, SR., et al. 1990. Mol Endocrinol. 4: 1069-80. PMID: 2284007
5. A Novel Clinical Entity of Autoimmune Endocrinopathy: Anti-PIT-1 Antibody Syndrome.  |  Iguchi, G., et al. 2017. Front Horm Res. 48: 76-83. PMID: 28245453
6. The impact of type III sodium-dependent phosphate transporters (Pit 1 and Pit 2) on podocyte and kidney function.  |  Kulesza, T. and Piwkowska, A. 2021. J Cell Physiol. 236: 7176-7185. PMID: 33738792
7. Cloning of a human GHF-1/Pit-1 cDNA variant.  |  Pernasetti, F., et al. 1993. Nucleic Acids Res. 21: 3584. PMID: 8346040
8. The transcription factor Pit-1/GHF-1 is expressed in hemopoietic and lymphoid tissues.  |  Delhase, M., et al. 1993. Eur J Immunol. 23: 951-5. PMID: 8458380
9. Interaction of Ets-1 and the POU-homeodomain protein GHF-1/Pit-1 reconstitutes pituitary-specific gene expression.  |  Bradford, AP., et al. 1997. Mol Cell Biol. 17: 1065-74. PMID: 9032233
10. Activities in Pit-1 determine whether receptor interacting protein 140 activates or inhibits Pit-1/nuclear receptor transcriptional synergy.  |  Chuang, FM., et al. 1997. Mol Endocrinol. 11: 1332-41. PMID: 9259323

The preferred Pit-1 antibody is Pit-1 Antibody (G-2): sc-25258.