pICln Antibody (G-1): sc-271327

pICln Antibody (G-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects pICln protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-pICln antibody (G-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. pICln protein plays a crucial role in the assembly of the spliceosome, a complex responsible for the processing of pre-mRNA into mature mRNA. This assembly involves the ordered incorporation of Sm proteins onto small nuclear RNAs (snRNAs), a process that is facilitated by the survival of motor neuron (SMN) protein and is significantly enhanced by the symmetrical dimethylation of specific arginine residues in Sm proteins. This post-translational modification is catalyzed by the methylosome, a complex that includes the type II methyltransferase PRMT5, pICln, and additional novel factors. The interaction between pICln and Sm proteins is vital, as pICln not only binds to the Sm domains but also acts as an inhibitor of small nuclear ribonucleoprotein (SnRNP) assembly, thereby regulating the formation of the Sm core. pICln is a highly conserved protein that is ubiquitously expressed and primarily localized in the cytoplasm, where pICln may also function as a swelling-activated anion channel or a channel regulator, highlighting its multifaceted role in cellular processes. The gene encoding human pICln is located on chromosome 11q14.1.

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pICln Antibody (G-1) References:

1. ICln is essential for cellular and early embryonic viability.  |  Pu, WT., et al. 2000. J Biol Chem. 275: 12363-6. PMID: 10777517
2. The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins.  |  Friesen, WJ., et al. 2001. Mol Cell Biol. 21: 8289-300. PMID: 11713266
3. Methylation of Sm proteins by a complex containing PRMT5 and the putative U snRNP assembly factor pICln.  |  Meister, G., et al. 2001. Curr Biol. 11: 1990-4. PMID: 11747828
4. Molecular cloning and expression of a chloride channel-associated protein pICln in human young red blood cells: association with actin.  |  Schwartz, RS., et al. 1997. Biochem J. 327 (Pt 2): 609-16. PMID: 9359436
5. Characterization of pI(Cln) binding proteins: identification of p17 and assessment of the role of acidic domains in mediating protein-protein interactions.  |  Emma, F., et al. 1998. Biochim Biophys Acta. 1404: 321-8. PMID: 9739160
6. Recombinant pICln forms highly cation-selective channels when reconstituted into artificial and biological membranes.  |  Li, C., et al. 1998. J Gen Physiol. 112: 727-36. PMID: 9834142

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pICln Antibody (G-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-pICln Antibody (G-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pICln Antibody (G-1) Specifications

Alternate Product Name: pICln

Product Clone Name: G-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-237

Epitope Target: pICln

Epitope Species: human

Product Reactivity/Detection: pICln

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for pICln Antibody (G-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin