PheRS Antibody (F-9): sc-166048
- PheRS Antibody (F-9) is a mouse monoclonal IgG1 κ PheRS antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of PheRS of human origin
- PheRS Antibody (F-9) is recommended for detection of PheRS of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PheRS Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PheRS Antibody (F-9) for WB applications. These reagents are now offered in bundles with PheRS Antibody (F-9) (see ordering information below).
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PheRS Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects PheRS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PheRS antibody (F-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PheRS protein, also known as phenylalanyl-tRNA synthetase 2, mitochondrial (FARS2), plays a critical role in mitochondrial protein synthesis by catalyzing the attachment of L-phenylalanine to its corresponding tRNA, a process essential for accurate translation and protein expression. PheRS operates within the mitochondrial matrix, ensuring correct amino acid incorporation into proteins, thereby maintaining cellular function and integrity. PheRS involvement in proper folding and function of mitochondrial proteins is vital for energy production and metabolic processes. The gene encoding PheRS is located on human chromosome 6, a region significant due to its size and number of genes, comprising nearly 6% of the human genome.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PheRS Antibody (F-9) References:
- Expression and characterization of a human mitochondrial phenylalanyl-tRNA synthetase. | Bullard, JM., et al. 1999. J Mol Biol. 288: 567-77. PMID: 10329163
- Phenylalanyl-tRNA synthetase contains a dispensable RNA-binding domain that contributes to the editing of noncognate aminoacyl-tRNA. | Roy, H. and Ibba, M. 2006. Biochemistry. 45: 9156-62. PMID: 16866361
- Structural and mutational studies of the amino acid-editing domain from archaeal/eukaryal phenylalanyl-tRNA synthetase. | Sasaki, HM., et al. 2006. Proc Natl Acad Sci U S A. 103: 14744-9. PMID: 17003130
- Purification, crystallization and preliminary X-ray characterization of a human mitochondrial phenylalanyl-tRNA synthetase. | Levin, I., et al. 2007. Acta Crystallogr Sect F Struct Biol Cryst Commun. 63: 761-4. PMID: 17768348
- Phenylalanyl-tRNA synthetase editing defects result in efficient mistranslation of phenylalanine codons as tyrosine. | Ling, J., et al. 2007. RNA. 13: 1881-6. PMID: 17804641
- The tRNA-induced conformational activation of human mitochondrial phenylalanyl-tRNA synthetase. | Klipcan, L., et al. 2008. Structure. 16: 1095-104. PMID: 18611382
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PheRS Antibody (F-9) | sc-166048 | 200 µg/ml | $322.00 | |||
PheRS Antibody (F-9): m-IgG Fc BP-HRP Bundle | sc-528898 | 200 µg Ab; 10 µg BP | $361.00 | |||
PheRS Antibody (F-9): m-IgGκ BP-HRP Bundle | sc-521496 | 200 µg Ab, 40 µg BP | $361.00 | |||
PheRS Antibody (F-9): m-IgG1 BP-HRP Bundle | sc-543200 | 200 µg Ab; 20 µg BP | $361.00 | |||
PheRS Antibody (F-9) AC | sc-166048 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PheRS Antibody (F-9) HRP | sc-166048 HRP | 200 µg/ml | $322.00 | |||
PheRS Antibody (F-9) FITC | sc-166048 FITC | 200 µg/ml | $336.00 | |||
PheRS Antibody (F-9) PE | sc-166048 PE | 200 µg/ml | $349.00 | |||
PheRS Antibody (F-9) Alexa Fluor® 488 | sc-166048 AF488 | 200 µg/ml | $364.00 | |||
PheRS Antibody (F-9) Alexa Fluor® 546 | sc-166048 AF546 | 200 µg/ml | $364.00 | |||
PheRS Antibody (F-9) Alexa Fluor® 594 | sc-166048 AF594 | 200 µg/ml | $364.00 | |||
PheRS Antibody (F-9) Alexa Fluor® 647 | sc-166048 AF647 | 200 µg/ml | $364.00 | |||
PheRS Antibody (F-9) Alexa Fluor® 680 | sc-166048 AF680 | 200 µg/ml | $364.00 | |||
PheRS Antibody (F-9) Alexa Fluor® 790 | sc-166048 AF790 | 200 µg/ml | $364.00 |