Peptide YY CRISPR/Cas9 KO Plasmid (h): sc-403446
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Peptide YY CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Peptide YY genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Peptide YY Antibody (029-01-1): sc-80499
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Peptide YY CRISPR/Cas9 KO Plasmid (h) | sc-403446 | 20 µg | $397.00 |
Overview
PYY encodes peptide YY, a gut-derived endocrine peptide secreted primarily by enteroendocrine L cells in response to nutrient intake and processed into bioactive forms that signal through neuropeptide Y receptor family members, notably Y2. Peptide YY modulates gastrointestinal motility, secretion, and satiety signaling within gut–brain axis circuits, integrating enteroendocrine cues with hypothalamic and vagal pathways. Through these receptor-mediated GPCR processes, PYY contributes to energy homeostasis and metabolic regulation, and altered expression patterns have been associated with obesity, type 2 diabetes, and gastrointestinal disorders. PYY is also used as a marker in studies of enteroendocrine differentiation and intestinal epithelial biology.
Peptide YY CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PYY gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PYY together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PYY open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Peptide YY protein expression.
This CRISPR knockout system enables efficient generation of PYY-deficient cell models for investigation of Peptide YY signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PYY exon(s) critical for Peptide YY function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PYY genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Peptide YY CRISPR/Cas9 KO Plasmid (h) and Peptide YY CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PYY locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Peptide YY HDR Plasmid (h) and Peptide YY HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PYY homology arms to support homology-directed repair at defined PYY target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.