PCGF5 Double Nickase Plasmid (h): sc-416432-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PCGF5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PCGF5 Double Nickase Plasmid (h) and PCGF5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PCGF5. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCGF5 Double Nickase Plasmid (h) | sc-416432-NIC | 20 µg | $410.00 |
PCGF5 (polycomb group ring finger 5) encodes a RING finger protein that associates with Polycomb group complexes and contributes to chromatin-dependent gene repression. Through regulation of histone modifications and higher-order chromatin architecture, PCGF5 helps control transcriptional programs involved in cell identity, differentiation, and developmental patterning. PCGF5 has been linked to Polycomb-mediated silencing pathways that intersect with cell cycle control and lineage specification, processes frequently perturbed in cancer and neurodevelopmental disease contexts. Its molecular interactions within PRC1-related assemblies make it a useful target for dissecting epigenetic regulation of gene networks in human cells.
PCGF5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PCGF5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PCGF5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PCGF5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PCGF5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.