Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
paxillin CRISPR/Cas9 KO Plasmid (h): sc-416733
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- paxillin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the paxillin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: paxillin Antibody (B-2): sc-365379
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
paxillin CRISPR/Cas9 KO Plasmid (h) | sc-416733 | 20 µg | $397.00 |
Overview
PXN encodes paxillin, a focal adhesion adaptor protein that coordinates integrin signaling with actin cytoskeleton remodeling to control cell adhesion, spreading, and migration. Paxillin functions as a scaffold for kinases and structural proteins including FAK and SRC, linking extracellular matrix cues to downstream pathways such as MAPK/ERK and PI3K signaling. Through regulation of focal adhesion turnover and mechanotransduction, paxillin contributes to processes including wound repair, immune cell trafficking, and extracellular matrix–driven cellular responses. Dysregulated paxillin signaling and focal adhesion dynamics are frequently investigated in the context of altered cell motility and invasion phenotypes relevant to cancer biology and fibrotic or inflammatory microenvironments.
paxillin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PXN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PXN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PXN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish paxillin protein expression.
This CRISPR knockout system enables efficient generation of PXN-deficient cell models for investigation of paxillin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PXN exon(s) critical for paxillin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PXN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by paxillin CRISPR/Cas9 KO Plasmid (h) and paxillin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PXN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by paxillin HDR Plasmid (h) and paxillin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PXN homology arms to support homology-directed repair at defined PXN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.