Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PARP1 Double Nickase Plasmid (m): sc-419018-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PARP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PARP1 Double Nickase Plasmid (m) and PARP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Parp1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PARP1 Antibody (B-10): sc-74470
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARP1 Double Nickase Plasmid (m) | sc-419018-NIC | 20 µg | $410.00 | |||
PARP1 Double Nickase Plasmid (m2) | sc-419018-NIC-2 | 20 µg | $410.00 |
Mouse Parp1 encodes poly(ADP-ribose) polymerase 1 (PARP1), a chromatin-associated DNA damage sensor that catalyzes PARylation of itself and other nuclear proteins in response to single- and double-strand breaks. PARP1 coordinates base excision repair and single-strand break repair and influences replication fork stability through recruitment of DNA repair factors and remodeling of chromatin structure. Beyond genome maintenance, PARP1 modulates transcriptional programs and inflammatory signaling, including crosstalk with NF-κB and stress-response pathways. Dysregulated PARP1 activity or altered Parp1 expression is linked to genomic instability and is frequently studied in contexts such as cancer biology, neurodegeneration, and ischemia-related tissue injury in mouse models.
PARP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Parp1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Parp1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Parp1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Parp1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.