Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PABP Double Nickase Plasmid (h): sc-400688-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PABP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PABP Double Nickase Plasmid (h) and PABP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PABPC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PABP Antibody (10E10): sc-32318
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PABP Double Nickase Plasmid (h) | sc-400688-NIC | 20 µg | $410.00 | |||
PABP Double Nickase Plasmid (h2) | sc-400688-NIC-2 | 20 µg | $410.00 |
Human PABPC1 encodes poly(A)-binding protein (PABP), a core RNA-binding factor that coats mRNA poly(A) tails and coordinates translation initiation, mRNA stabilization, and deadenylation-dependent turnover. Through interactions with eIF4G and other translation machinery, PABP promotes mRNA circularization and regulates protein synthesis rates, while also influencing miRNA-mediated silencing and stress granule dynamics. PABP-dependent control of transcript fate links PABPC1 to pathways governing cell growth, stress responses, and RNA quality control. Dysregulated RNA metabolism and translational control implicate PABPC1-associated processes in cancer biology and neurodegeneration-relevant phenotypes, where altered mRNA stability and proteostasis are common features.
PABP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PABPC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PABPC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PABPC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PABPC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.