p38IP Double Nickase Plasmid (h): sc-409288-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p38IP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p38IP Double Nickase Plasmid (h) and p38IP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SUPT20H. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p38IP Antibody (C-7): sc-374665
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p38IP Double Nickase Plasmid (h) | sc-409288-NIC | 20 µg | $410.00 |
SUPT20H encodes p38IP, a conserved adaptor that associates with MAPK p38 signaling and chromatin regulatory machinery to coordinate stress-responsive transcription. p38IP has been linked to modulation of MAPK pathway outputs and regulation of gene expression programs that influence cell-cycle progression, differentiation, and inflammatory responses. Through these functions, SUPT20H/p38IP contributes to cellular homeostasis under genotoxic and environmental stress and is studied in contexts where dysregulated MAPK signaling and transcriptional control are implicated in disease-relevant phenotypes, including altered proliferation and immune signaling.
p38IP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SUPT20H locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SUPT20H. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SUPT20H function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SUPT20H-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.