p-MEK-1 Antibody (B-4): sc-271914
- p-MEK-1 Antibody (B-4) is a mouse monoclonal IgG1 κ, cited in 14 publications, provided at 200 µg/ml
- raised against a short amino acid sequence containing phosphorylated Ser 298 of MEK1 of human origin
- Anti-p-MEK-1 Antibody (B-4) is recommended for detection of Ser 298 phosphorylated MEK1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-p-MEK-1 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for p-MEK-1 Antibody (B-4) for WB applications. This reagent is now offered in a bundle with p-MEK-1 Antibody (B-4) (see ordering information below).
p-MEK-1 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 298 phosphorylated MEK1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-MEK-1 (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MEK1, a crucial member of the MAP kinase kinase family, plays a vital role in the MAPK signaling pathway by specifically phosphorylating ERK, which is essential for cell proliferation, differentiation, and survival. Phosphorylation of MEK1 at Ser 298 enhances kinase activity, facilitating activation of downstream MAP kinases. This regulatory mechanism enables proper transmission of extracellular signals to the nucleus, influencing various cellular responses. Understanding p-MEK-1 phosphorylation state helps elucidate signal transduction pathways and their implications in diseases such as cancer, where aberrant signaling can lead to uncontrolled cell growth.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-MEK-1 Antibody (B-4) References:
- The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. | Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
- Theophylline enhances melanogenesis in B16F10 murine melanoma cells through the activation of the MEK 1/2, and Wnt/β-catenin signaling pathways. | Huang, HC., et al. 2020. Food Chem Toxicol. 137: 111165. PMID: 32001318
- Components of a new human protein kinase signal transduction pathway. | Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
- Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms. | Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
- Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2. | Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
- Characterization of the structure and function of a novel MAP kinase kinase (MKK6). | Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
- Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta). | Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
- Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase. | Tournier, C., et al. 1997. Proc Natl Acad Sci U S A. 94: 7337-42. PMID: 9207092
- MKK7 is a stress-activated mitogen-activated protein kinase kinase functionally related to hemipterous. | Holland, PM., et al. 1997. J Biol Chem. 272: 24994-8. PMID: 9312105
- Molecular cloning and characterization of human JNKK2, a novel Jun NH2-terminal kinase-specific kinase. | Wu, Z., et al. 1997. Mol Cell Biol. 17: 7407-16. PMID: 9372971
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-MEK-1 Antibody (B-4) | sc-271914 | 200 µg/ml | $322.00 | |||
p-MEK-1 Antibody (B-4): m-IgG Fc BP-HRP Bundle | sc-525837 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-MEK-1 Antibody (B-4) AC | sc-271914 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-MEK-1 Antibody (B-4) HRP | sc-271914 HRP | 200 µg/ml | $322.00 | |||
p-MEK-1 Antibody (B-4) FITC | sc-271914 FITC | 200 µg/ml | $336.00 | |||
p-MEK-1 Antibody (B-4) PE | sc-271914 PE | 200 µg/ml | $349.00 | |||
p-MEK-1 Antibody (B-4) Alexa Fluor® 488 | sc-271914 AF488 | 200 µg/ml | $364.00 | |||
p-MEK-1 Antibody (B-4) Alexa Fluor® 546 | sc-271914 AF546 | 200 µg/ml | $364.00 | |||
p-MEK-1 Antibody (B-4) Alexa Fluor® 594 | sc-271914 AF594 | 200 µg/ml | $364.00 | |||
p-MEK-1 Antibody (B-4) Alexa Fluor® 647 | sc-271914 AF647 | 200 µg/ml | $364.00 | |||
p-MEK-1 Antibody (B-4) Alexa Fluor® 680 | sc-271914 AF680 | 200 µg/ml | $364.00 | |||
p-MEK-1 Antibody (B-4) Alexa Fluor® 790 | sc-271914 AF790 | 200 µg/ml | $364.00 | |||
p-MEK-1 (B-4) Neutralizing Peptide | sc-271914 P | 100 µg/0.5 ml | $69.00 |