Oct-1 Antibody (YL15): sc-53830

Oct-1 Antibody (YL15) is a mouse monoclonal IgG1 antibody that detects Oct-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Oct-1, a member of the POU domain family of transcription factors, plays a crucial role in regulating gene expression by binding to specific DNA sequences known as octamer motifs, which are vital for the transcription of various genes, including those involved in immune responses and cellular differentiation. Oct-1′s ability to form dimers, particularly with the transcriptional co-activator OBF1, enhances regulatory capacity, allowing Oct-1 to influence tissue-specific gene expression. This is particularly important in the context of immunoglobulin gene transcription, where Oct-1′s interaction with octamer elements is essential for proper activation of immunoglobulin promoters. Oct-1 is ubiquitously expressed across different cell types, highlighting a fundamental role in maintaining cellular functions and responding to developmental cues. With versatile binding capabilities and regulatory functions, Oct-1 is integral to transcriptional machinery, making Oct-1 (YL15) antibody an invaluable tool for researchers studying gene regulation and expression in various biological contexts.

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Oct-1 Antibody (YL15) References:

1. T to C substitution at -175 or -173 of the gamma-globin promoter affects GATA-1 and Oct-1 binding in vitro differently but can independently reproduce the hereditary persistence of fetal hemoglobin phenotype in transgenic mice.  |  Liu, LR., et al. 2005. J Biol Chem. 280: 7452-9. PMID: 15613485
2. Oct-1 acts as a transcriptional repressor on the C-reactive protein promoter.  |  Voleti, B., et al. 2012. Mol Immunol. 52: 242-8. PMID: 22750226
3. Transcriptional regulation of the HMGA1 gene by octamer-binding proteins Oct-1 and Oct-2.  |  Chiefari, E., et al. 2013. PLoS One. 8: e83969. PMID: 24367622
4. OCT1 in hepatic steatosis and thiamine disposition.  |  Chen, L., et al. 2015. Cell Cycle. 14: 283-4. PMID: 25590948
5. Oct-2 forms a complex with Oct-1 on the iNOS promoter and represses transcription by interfering with recruitment of RNA PolII by Oct-1.  |  Bentrari, F., et al. 2015. Nucleic Acids Res. 43: 9757-65. PMID: 26271992
6. Transcription factor organic cation transporter 1 (OCT-1) affects the expression of porcine Klotho (KL) gene.  |  Li, Y., et al. 2016. PeerJ. 4: e2186. PMID: 27478698
7. Genetic polymorphisms of organic cation transporter 1 (OCT1) and responses to metformin therapy in individuals with type 2 diabetes: A systematic review.  |  Mofo Mato, EP., et al. 2018. Medicine (Baltimore). 97: e11349. PMID: 29979413
8. OBF1 and OCT1/2 regulate the germinal center B-cell program.  |  Küppers, R. 2021. Blood. 137: 2862-2863. PMID: 34042984
9. Suppression of OCT-1 in Metastatic Breast Cancer Cells Reduces Tumor Metastatic Potential, Hypoxia Resistance, and Drug Resistance.  |  Stepchenko, AG., et al. 2022. Life (Basel). 12: PMID: 36143471
10. Using altered specificity Oct-1 and Oct-2 mutants to analyze the regulation of immunoglobulin gene transcription.  |  Shah, PC., et al. 1997. EMBO J. 16: 7105-17. PMID: 9384588

The preferred Oct-1 antibody is Oct-1 Antibody (12F11): sc-8024.