OB-cadherin CRISPR/Cas9 KO Plasmid (h): sc-401051
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OB-cadherin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OB-cadherin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OB-cadherin Antibody (F-3): sc-365867
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OB-cadherin CRISPR/Cas9 KO Plasmid (h) | sc-401051 | 20 µg | $397.00 |
Overview
CDH11 encodes OB-cadherin, a classical cadherin that mediates calcium-dependent homophilic cell–cell adhesion and contributes to tissue architecture and intercellular signaling. Through interactions with catenins, OB-cadherin links adherens junctions to the actin cytoskeleton and influences pathways governing cell polarity, migration, and mechanotransduction, including regulation of β-catenin availability and junctional remodeling. CDH11 is prominently expressed in mesenchymal lineages and stromal compartments, where it supports extracellular matrix organization and cell motility programs. Dysregulated CDH11 expression has been associated with fibrotic remodeling, inflammatory joint pathology, and tumor–stroma interactions relevant to invasion and metastatic niche formation.
OB-cadherin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDH11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDH11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDH11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OB-cadherin protein expression.
This CRISPR knockout system enables efficient generation of CDH11-deficient cell models for investigation of OB-cadherin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDH11 exon(s) critical for OB-cadherin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDH11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OB-cadherin CRISPR/Cas9 KO Plasmid (h) and OB-cadherin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDH11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OB-cadherin HDR Plasmid (h) and OB-cadherin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDH11 homology arms to support homology-directed repair at defined CDH11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.