Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nup50 CRISPR/Cas9 KO Plasmid (h): sc-406550
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nup50 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nup50 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nup50 Antibody (G-4): sc-398993
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nup50 CRISPR/Cas9 KO Plasmid (h) | sc-406550 | 20 µg | $397.00 |
Overview
NUP50 encodes Nup50, a dynamic component of the nuclear pore complex that participates in nucleocytoplasmic transport by modulating importin-α/β–dependent cargo trafficking and recycling within the nucleus. Through its interactions at the nuclear basket, Nup50 helps coordinate nuclear import kinetics with broader processes such as cell-cycle progression, transcriptional regulation, and maintenance of nuclear architecture. Disruption of nuclear transport pathways and nuclear pore complex homeostasis is frequently implicated in genome instability and altered signaling programs relevant to cancer biology and other disorders characterized by impaired nucleocytoplasmic compartmentalization. Accordingly, NUP50 is commonly studied in the context of nuclear transport regulation, proteostasis, and stress-responsive signaling.
Nup50 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NUP50 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NUP50 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NUP50 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nup50 protein expression.
This CRISPR knockout system enables efficient generation of NUP50-deficient cell models for investigation of Nup50 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NUP50 exon(s) critical for Nup50 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NUP50 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nup50 CRISPR/Cas9 KO Plasmid (h) and Nup50 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NUP50 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nup50 HDR Plasmid (h) and Nup50 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NUP50 homology arms to support homology-directed repair at defined NUP50 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.