Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nox5 Double Nickase Plasmid (h): sc-401223-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nox5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nox5 Double Nickase Plasmid (h) and Nox5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NOX5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nox5 Antibody (A-3): sc-518114
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nox5 Double Nickase Plasmid (h) | sc-401223-NIC | 20 µg | $410.00 | |||
Nox5 Double Nickase Plasmid (h2) | sc-401223-NIC-2 | 20 µg | $410.00 |
NOX5 encodes Nox5, a calcium-activated NADPH oxidase that generates reactive oxygen species (ROS) as signaling intermediates in human cells. By coupling intracellular Ca2+ dynamics to regulated superoxide and hydrogen peroxide production, Nox5 influences redox-sensitive pathways including MAPK, NF-κB, and modulation of ion channels and cytoskeletal remodeling. Nox5-derived ROS contribute to control of proliferation, migration, and inflammatory responses, with dysregulation implicated in oxidative stress–associated phenotypes relevant to cardiovascular and metabolic biology. Its activity is frequently studied in the context of endothelial and smooth muscle signaling, immune regulation, and redox-dependent transcriptional programs.
Nox5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NOX5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NOX5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NOX5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NOX5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.