Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Double Nickase Plasmid (h): sc-401605-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Double Nickase Plasmid (h) and Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHRNA4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Antibody (A-6): sc-74519
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Double Nickase Plasmid (h) | sc-401605-NIC | 20 µg | $410.00 | |||
Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Double Nickase Plasmid (h2) | sc-401605-NIC-2 | 20 µg | $410.00 |
CHRNA4 encodes the α4 subunit of neuronal nicotinic acetylcholine receptors (nAChRs), which assemble as pentameric ligand-gated cation channels commonly with β2 subunits to form high-affinity α4β2 receptors. Upon acetylcholine or nicotine binding, these receptors mediate Na⁺ and Ca²⁺ influx to regulate membrane excitability, synaptic transmission, and activity-dependent plasticity in cholinergic circuits. CHRNA4-dependent signaling intersects with calcium-dependent pathways and neurotransmitter release programs that shape attention, arousal, and reward-related neuronal network function. Genetic and functional perturbations in CHRNA4 have been associated with neuropsychiatric and neurological phenotypes, making it a relevant target for mechanistic studies of cholinergic signaling and receptor biology.
Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHRNA4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHRNA4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHRNA4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHRNA4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.