Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NG2 Double Nickase Plasmid (h): sc-400699-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NG2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NG2 Double Nickase Plasmid (h) and NG2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSPG4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NG2 Antibody (LHM 2): sc-53389
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NG2 Double Nickase Plasmid (h) | sc-400699-NIC | 20 µg | $410.00 | |||
NG2 Double Nickase Plasmid (h2) | sc-400699-NIC-2 | 20 µg | $410.00 |
CSPG4 encodes the transmembrane chondroitin sulfate proteoglycan NG2, a multifunctional co-receptor that modulates cell–matrix interactions and growth factor signaling. NG2 influences cytoskeletal remodeling, adhesion, and migration through pathways linked to integrin/FAK, PDGF, and MAPK/ERK signaling, supporting proliferative and invasive cellular phenotypes. In human tissues, CSPG4/NG2 expression is associated with progenitor-like states and perivascular or stromal programs, and it is frequently studied in the context of tumor biology, angiogenic remodeling, and therapy resistance mechanisms. Dysregulated NG2 signaling and extracellular matrix engagement are therefore relevant to research on malignant progression, vascular niche dynamics, and microenvironment-driven plasticity.
NG2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSPG4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSPG4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSPG4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSPG4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.