Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NFS1 Double Nickase Plasmid (h): sc-403102-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NFS1 Double Nickase Plasmid (h) and NFS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NFS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFS1 Antibody (B-7): sc-365308
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFS1 Double Nickase Plasmid (h) | sc-403102-NIC | 20 µg | $410.00 | |||
NFS1 Double Nickase Plasmid (h2) | sc-403102-NIC-2 | 20 µg | $410.00 |
NFS1 encodes a cysteine desulfurase that initiates de novo iron–sulfur (Fe–S) cluster biogenesis in mitochondria by mobilizing sulfur from L-cysteine and transferring it to the ISC assembly machinery. Through this pathway, NFS1 supports maturation of Fe–S–dependent enzymes involved in oxidative phosphorylation, lipoic acid metabolism, and maintenance of mitochondrial and nuclear genome integrity. Perturbation of NFS1 can disrupt mitochondrial respiration and redox homeostasis, with downstream effects on cell proliferation and stress responses. Genetic and functional studies link impaired Fe–S cluster assembly to multisystem mitochondrial disease phenotypes, making NFS1 a key target for mechanistic investigations of metabolic vulnerability and genome maintenance.
NFS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NFS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NFS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NFS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NFS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.