Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NF45 Double Nickase Plasmid (h): sc-402042-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NF45 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NF45 Double Nickase Plasmid (h) and NF45 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ILF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NF45 Antibody (H-4): sc-365283
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NF45 Double Nickase Plasmid (h) | sc-402042-NIC | 20 µg | $410.00 | |||
NF45 Double Nickase Plasmid (h2) | sc-402042-NIC-2 | 20 µg | $410.00 |
Human ILF2 encodes NF45, an RNA- and DNA-binding factor that forms a functional heterodimer with ILF3/NF90 to regulate transcription, RNA processing, mRNA stability, and translation. NF45 participates in cellular stress responses and immune-associated gene expression programs, with reported roles in controlling cell-cycle progression and maintaining genome integrity through its influence on nucleic acid metabolism. Through these activities, ILF2/NF45 is frequently studied in pathways linked to proliferation and apoptosis, and its dysregulation has been associated with tumor biology and altered responses to genotoxic stress. NF45 is also examined in the context of antiviral and inflammatory signaling where post-transcriptional control of cytokine-related transcripts is important.
NF45 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ILF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ILF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ILF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ILF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.