NF-H Antibody (2D2): sc-20014

NF-H Antibody (2D2) is a mouse monoclonal IgM antibody that detects neurofilament-H (NF-H) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). NF-H is a crucial component of the neuronal cytoskeleton, belonging to the intermediate filament family, and plays a significant role in maintaining the structural integrity of neurons. NF-H′s dynamic nature is underscored by numerous phosphorylation sites, which are targeted by various protein kinases, including protein kinase A, protein kinase C, and cyclin-dependent kinase 5. These phosphorylation events are vital for regulating neurofilament assembly and disassembly, which in turn influences axon caliber and overall neuronal function. Alterations in NF-H phosphorylation and metabolism are often linked to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, making NF-H a key biomarker for studying these conditions. Anti-NF-H antibody (2D2) provides researchers with a powerful tool for investigating the role of neurofilaments in neuronal health and disease, facilitating a deeper understanding of the underlying mechanisms of neurodegeneration.

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NF-H Antibody (2D2) References:

1. Neurofilament proteins NF-L, NF-M and NF-H in brain of patients with Down syndrome and Alzheimer's disease.  |  Bajo, M., et al. 2001. Amino Acids. 21: 293-301. PMID: 11764410
2. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury.  |  Shaw, G., et al. 2005. Biochem Biophys Res Commun. 336: 1268-77. PMID: 16176808
3. Hyperphosphorylated neurofilament NF-H as a predictor of mortality after brain injury in children.  |  Zurek, J., et al. 2011. Brain Inj. 25: 221-6. PMID: 21219092
4. The C-terminal domains of NF-H and NF-M subunits maintain axonal neurofilament content by blocking turnover of the stationary neurofilament network.  |  Rao, MV., et al. 2012. PLoS One. 7: e44320. PMID: 23028520
5. Phosphorylated neurofilament subunit NF-H becomes elevated in the cerebrospinal fluid of patients with acutely worsening symptoms of compression myelopathy.  |  Takahashi, H., et al. 2014. J Clin Neurosci. 21: 2175-8. PMID: 25065845
6. Expression of subtypes NF-L, NF-M and NF-H of neurofilament triplet proteins in the developing rat inner ear.  |  Nishizaki, K. and Anniko, M. 1995. ORL J Otorhinolaryngol Relat Spec. 57: 177-81. PMID: 7478449
7. Increasing neurofilament subunit NF-M expression reduces axonal NF-H, inhibits radial growth, and results in neurofilamentous accumulation in motor neurons.  |  Wong, PC., et al. 1995. J Cell Biol. 130: 1413-22. PMID: 7559762
8. Neuronal cyclin-dependent kinase-5 phosphorylation sites in neurofilament protein (NF-H) are dephosphorylated by protein phosphatase 2A.  |  Veeranna,., et al. 1995. J Neurochem. 64: 2681-90. PMID: 7760048
9. Neurofilament subunit NF-H modulates axonal diameter by selectively slowing neurofilament transport.  |  Marszalek, JR., et al. 1996. J Cell Biol. 135: 711-24. PMID: 8909545
10. Neurofilament-dependent radial growth of motor axons and axonal organization of neurofilaments does not require the neurofilament heavy subunit (NF-H) or its phosphorylation.  |  Rao, MV., et al. 1998. J Cell Biol. 143: 171-81. PMID: 9763429

The preferred NF-H antibody is NF-H Antibody (RNF402): sc-32729.