Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Neurogenin 3 CRISPR/Cas9 KO Plasmid (m): sc-419234
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neurogenin 3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Neurogenin 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Neurogenin 3 Antibody (C-7): sc-374442
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neurogenin 3 CRISPR/Cas9 KO Plasmid (m) | sc-419234 | 20 µg | $397.00 |
Overview
Mouse Neurog3 encodes the basic helix–loop–helix transcription factor Neurogenin 3, a key determinant of endocrine lineage specification in the developing pancreas and gut. Neurogenin 3 integrates developmental signaling inputs, including Notch/Hes-mediated repression and pro-endocrine transcriptional cascades, to drive endocrine progenitor commitment and subsequent differentiation programs. Its activity coordinates gene regulatory networks controlling cell fate decisions, maturation, and hormone-producing cell identity, making Neurog3 a central node for studies of pancreatic development and endocrine cell biology. Dysregulated Neurog3 function is relevant to models of impaired endocrine differentiation and metabolic disease mechanisms involving pancreatic islet formation.
Neurogenin 3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Neurog3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Neurog3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Neurog3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Neurogenin 3 protein expression.
This CRISPR knockout system enables efficient generation of Neurog3-deficient cell models for investigation of Neurogenin 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Neurog3 exon(s) critical for Neurogenin 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Neurog3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Neurogenin 3 CRISPR/Cas9 KO Plasmid (m) and Neurogenin 3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Neurog3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Neurogenin 3 HDR Plasmid (m) and Neurogenin 3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Neurog3 homology arms to support homology-directed repair at defined Neurog3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.