NAIP Double Nickase Plasmid (h): sc-418189-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NAIP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NAIP Double Nickase Plasmid (h) and NAIP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NAIP. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NAIP Double Nickase Plasmid (h) | sc-418189-NIC | 20 µg | $410.00 | |||
NAIP Double Nickase Plasmid (h2) | sc-418189-NIC-2 | 20 µg | $410.00 |
Human NAIP (neuronal apoptosis inhibitory protein) is a member of the NLR family that functions as an innate immune sensor and regulator of inflammasome signaling. NAIP recognizes bacterial ligands and cooperates with NLRC4 to promote inflammasome assembly, leading to caspase-1 activation and downstream maturation of IL-1β and IL-18, with broader impacts on pyroptosis and inflammatory homeostasis. Through these pathways, NAIP influences host defense, epithelial barrier responses, and immune cell activation programs. Dysregulated inflammasome activity and altered NAIP expression have been linked to inflammatory disorders and tumor-associated inflammation, making NAIP a useful node for studying disease-relevant immune signaling networks.
NAIP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NAIP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NAIP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NAIP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NAIP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.